Leonardo Da Vinci was spending his whole life for scientific research and artistic practice, the "Mona Lisa" reflected the painter's observation of nature in a scientific thinking, which was the most prestigious portrait masterpiece in the history of western oil painting. In this painting, Mona Lisa looked very elegant and calm with a meaningful smile. The painter sought to demonstrate the richness of life and multifaceted personality psychology, and studied the smiles of facial muscles and psychological changes and reflections when relaxing from two aspects: anatomy and physiology. He depicted an ideal beauty in a specific character. The hands were portrayed delicate, rich and dynamic. Eyes, mouth and hands were ingeniously combined with soft shadows. The background was also treated properly with difference on the left and right perspective, making the whole picture more vivid.
There was less agreement among different people on the mysterious smile of Mona Lisa for 500 years. Harvard Medical School neurobiologist Margaret Livingstone explained in U.S.Science magazine, "The mysterious smile of Mona Lisa is less concerned with the painter's painting techniques, than it is related to the dislocation of the eyes. Her flickering smiles are resulting from our eyes movements combining with the reflection of light. If our attention were solely focused on her mouth, then we would never see her smile. Da Vinci made the use of vague outline in the mouth of Mona Lisa. This approach meant "evaporated like smoke" according to the original Italian literal meaning."
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